TWO-DIMENSIONAL IMMUNOELECTROPHORESIS IN AGAR
نویسندگان
چکیده
منابع مشابه
Rocket and Two Dimensional Immunoelectrophoresis in Diagnosis of Caprine Brucellosis
BACKGROUND Brucellosis is a major bacterial zoonosis of global importance with the causative organisms of Gram-negative facultative intracellular pathogens. The aims of this study were to standardize two immunoelectrophoretic techniques, rocket and cross immunoelectrophoresis, and compare their results with other conventional serodiagnostic tests. METHODS Sera from 15 sheep, without any histo...
متن کاملCharacterization and comparison of mycobacterial antigens by two-dimensional immunoelectrophoresis.
Two-dimensional immunoelectrophoresis (2D-IEP), in which a complex of antigens is subjected to electrophoresis first through an agarose matrix in one direction and secondly through an antiserum-agarose matrix at right angles to the first direction, was evaluated as a tool for analysis of mycobacterial antigens. Cell extracts from four species of mycobacteria, Mycobacterium tuberculosis (four st...
متن کاملRapid two-dimensional immunoelectrophoresis of human serum proteins.
We describe two versions of a two-dimensional immunoelectrophoretic system and illustrate their use for separating and estimating human serum proteins. Each is sensitive, rapid, and simple to perform, and requires no special apparatus and only 0.1-0.6 ml of antiserum. Both procedures appear to be potentially useful in the clinical laboratory. Time required to obtain a stained pattern varies fro...
متن کاملModifications to the technique of two-dimensional immunoelectrophoresis.
In recent years a number of sensitive immunological techniques have been developed to study proteins in biological fluids. The most recently described is that of Minchin Clarke & Freeman (1966) which is a variant of an immunoelectrophoretic method originally described by Ressler (1960) and given the name of crossed immunoelectrophoresis by Laurel1 (1966). It is highly sensitive and by using a c...
متن کاملrocket and two dimensional immunoelectrophoresis in diag-nosis of caprine brucellosis
background : brucellosis is a major bacterial zoonosis of global importance with the causative organisms of gram-negative facultative intracellular pathogens. the aims of this study were to standardize two immunoelectrophoretic techniques, rocket and cross immunoelectrophoresis, and compare their results with other conventional serodiagnostic tests. methods : sera from 15 sheep, without any his...
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ژورنال
عنوان ژورنال: Japanese Journal of Medical Science and Biology
سال: 1962
ISSN: 0021-5112,1884-2828
DOI: 10.7883/yoken1952.15.1